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Assessment of Genetic Variations among Medicinal Plant Cassia tora from Different Geographic Regions of Central India Using RAPD Markers

Anita Tilwari, Deepak Chauhan, Rajesh Sharma and RK Singh

In this study 13 different accessions of medicinal and aromatic plant Cassia tora obtained from different agro climatic zones of Madhya Pradesh (Central India) were evaluated for its genetic variability using the random amplified polymorphic DNA (RAPD) markers. During RAPD analysis, amplification of genomic DNA of the 13 accessions by 10 selected primers yielded 88 bright and discernible bands, of which 86 were polymorphic with an average of 9.6 bands per primer and polymorphism obtained was 97.72%. The amplified products varied in size from 2,111 to 153 bp. The Jaccard similarity coefficient value ranged from 0.23 to 0.65 indicate that the distribution of variation is diverse. A dendrogram constructed based on the UPGMA clustering method revealed three major clusters, which are again divided into sub clusters. The result revealed rich genetic diversity among Cassia tora accessions obtained from different agro climatic zones of central India, which gives its suitability to grow and conserve anywhere at any environmental conditions.