Mykobakterielle Erkrankungen
Offener Zugang

Abstrakt

Bioluminescence as tool to environmental contamination detection

Abdul Rhman H Muhammad

Recombinant bioluminescent bacteria are frequently directed towards use as environmental biosensors because it has high sensitivity, selectivity, costless, easy to use and function as rapid measurement to detect heavy metals. The bioluminescence of DF4/PUTK2 assay is based on using the bio-reporter Acinetobacter DF4/PUTK2 carrying luciferase genes luxCDABE which emit light constitutively. This can be measured in time intervals by luminometer to determine the behavior of bio-reporter against lead. The light emitted in the lead treated samples was equal or increased than the control. Therefore, the bio-reporter DF4/PUTK2 was subjected to intensive studies to elucidate its behavior with lead and if it was possible to be employed as a lead light-on assay in water in reverse order.