Klinische Pädiatrie: Offener Zugang
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Abstrakt

Circulating Y-RNAs: A Predicted Function Mainly in Controlling the Innate Immune System, Cell Signaling and DNA Replication in Pediatric Patients with Pilocytic and Diffuse Astrocytoma

Juan Manuel Rodríguez-Corona, Ruth Ruiz Esparza-Garrido, Jorge Víctor Horta Vega Miguel, Angel Velazquez Flores

Background: Y-RNAs are small noncoding RNAs firstly identified in patients suffering the Sjogren’s syndrome and lupus erythematosus, having different predicted cellular functions. Y-RNAs are very abundant ncRNAs present in serum and only a few is known respect their molecular roles.

Objective: The aim of the study was to determine the circulating Y-RNAs expression from blood serum of pediatric patients with pilocytic or diffuse astrocytoma.

Materials and methods: Y-RNAs expression was determined by means of the Human Transcriptome Array (HTA) 2.0 arrays. In addition, a bioinformatic approximation of the possible biological functions of Y-RNAs was determined with the Random Forest (RF) and Vector Support Machine (VSM) algorithms.

Results: Data showed a differential expression of RNY-3, RNY-4, and RNY-5 in pediatric patients with astrocytoma, relative to the control. Meanwhile, RNY-1 was shown to be upregulated in the diffuse condition versus the pilocytic. The bioinformatic analysis showed that RNY-4 and RNY-5 had the highest scores of interaction with Claudin, Toll-like receptors, and Hyaluronidase-1. In addition, the Y-RNAs loop domain by itself had the highest score of interaction with B cell receptor CD22 (RNY-3) and Toll-like receptor 7 and 3 (RNY-4).

Conclusion: Our results showed, for the first time a differential expression of circulating Y-RNAs in pediatric astrocytoma, allowing to distinguish pediatric subjects without cancer from patients with pediatric diffuse or pilocytic astrocytoma and their potential involvement in regulating diverse biological processes, such as immune activation-suppression, cell signaling, selective transcription, and cell proliferation through activation of DNA replication.