Fortgeschrittene Techniken in Biologie und Medizin
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Double-Staining Immunohistochemistry of Stem Cell Markers in Human Liver Cancer Tissues

Hiroyuki Tomita*, Kaori Tanaka, Kyoko Takahashi, Ayako Suga and Akira Hara

Recently, Immunohistochemistry (IHC) using specific antibodies has been exploited to detect the localization of stem cells and cancer stem cells (CSCs). Typically, there is a very small population of stem cells in normal and cancerous tissues, making it difficult to investigate their cell type and localization in human specimens. Hence, for pathological detection of human specimens, IHC has certain advantages over immunofluorescence staining. In the present study, we established a method for double-staining cancer stem cell markers and other putative markers associated with human liver cancer. We have summarized and reviewed the methodology for IHC with doublestaining in human liver cancer tissues.