Zeitschrift für Proteomik und Bioinformatik
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In Vitro and In Vivo Evaluation of Bauhinia variegata Extracts to Prevent Coxsackievirus B3 Infection

Mohamed Shaheen, Maria Borsanyiova, Sami Mostafa, Shubhada Bopegamage and Nagwa El-Esnawy

Coxsackievirus B3 (CVB3) is one of the most viral agents that cause myocarditis in human, particularly in infants and young children. However, up to date, there are no vaccine or antiviral agents to prevent and/or treat the disease caused by this virus. The aim of this study was to determine the antiviral activities of Bauhinia variegata extracts against CVB3 infection in vitro and in vivo. Five extracts from B. variegate leaves were tested for their antiviral activity against CVB3 in vitro by applying three different strategies using MTT and TCID50 assays. The antiviral activity in vivo was performed by monitoring of morbidity, mortality, the heart index, virus titers, and pathologic scores. In addition, measuring the activities of Aspartate Transaminase (AST), Creatine Kinase (CK), and Lactic Dehydrogenase (LDH) enzymes in infected mice with CVB3. Our results suggested that the methanol extract had the highest impact on viral infection in vitro as compared to others and it may work via blocking of the viral receptors. Moreover, this extract reduced the morbidity and mortality, the virus titers, and pathological area in the heart tissues of infected mice. Also, it maintained AST, CK and LDH enzymes at normal levels in the sera of the infected mice, when compared with infected control. In conclusion, the methanol extract of B. variegata leaves may play potential role in the treatment of CVB3 infection.