Zeitschrift für Chromatographie und Trenntechniken
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Exploring the Concept of Hydrotrophy in HPLC; Hydrotrophic Solutions as Novel Cost Effective, Eco-friendly and safe Mobile Phase for Method development and validation of Furosemide

Ahammed unais VP, Sahoo PK

Hydrotrophy is a unique molecular phenomenon that possess the power to extend the solubility of sparingly soluble and poorly soluble drugs in water. A hydrotrophic solution is defined as adding a solute, that is Hydrotrope, (eg: urea, Nicotinamide, Sodium benzoate, Sodium citrate etc.) to the solvent and is used as mobile phase for RP-HPLC. This concept minimizes the use of harmful and costly oragnic solvents to a larger extend which makes drugs analysis ecofriendly and less expensive. The methods was developed using 3% sodium benzoate (pH 5.5) as single mobile phase to review and conclude this subject. Welch ultisil ODS-3(C18) coloumn is used as stationary phase. The flow rate was maintaibed at 1 ml/min and detection was performed at 233 nm using DAD detector. The retension time was 4.5 min and method was detected to be linear over a concentration range of 25-100 microgram/ml with correlation coefficients of 0.9990. The developed method was well validated as per ICH Q2 (R1) guidelines and thereby confirmed that the proposed method can be applied for the routine analysis of furosemide in bulk and other pharmaceutical dosage forms and the objectives of the study are successfully achieved.