Chemotherapie: Offener Zugang
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Abstrakt

Expression of Classical Embryonic Stem Cell Markers in Pancreatic Cancer Cells

Hussain R Al-Turaifi

Background: Pancreatic cancer has been the third leading cause of cancer-related death in USA. Most of the cancer patients diagnosed in late stage which minimize the effectiveness of surgical intervention to less than 20 percentage. Moreover, chemo-radio therapy is not curative. Presence of Cancer Stem Cells (CSCs) within pancreatic tumor was reported by several groups using nonspecific biomarkers. Pluripotent transcription factors such as OCT4, SOX2 and NANOG, which are upregulated in embryonic stem cells in contrast to somatic cells, were detected in various type of cancer tumours from adult patients.
The aims of this study were to investigate the expression of the classical stem cell markers in Pancreatic Adenocarcinoma Cell line (PANC1).
Methods: PANC1 cells were characterised by RT-PCR/immuno-staining. Transient over-expression of stem cell promoter-driven reporter plasmid Oct4-eGFP was undertaken using Lipofectamine 2000 transfection reagent.
Results: Embryonic stem cell markers and other cancer related markers were detected which illustrate the nature of pancreatic cancer.
Conclusion: Embryonic stem cell markers could be used to identify pancreatic cancer stem cells and they are potential targets for cancer targeted therapy.