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In Vitro Regulation of Non-enzymatic Antioxidant Efficiency of Momordica dioica Roxb by Elicitation and Salt Stress

Raka Kamal, Pawan K. Katariya, Manas Mathur and Sunita Yadav

Antioxidants play an important role in inhibiting the free radicals thereby provides protection against infection and degenerative diseases. Natural antioxidants are scavenging reactive oxygen species (ROS), they are preferred over synthetic antioxidants because of their safety over the synthetic antioxidant chemicals. Momordica dioica Roxb., have been used in Indian traditional medicine for various ailments like antiallergic, hepatoprotective , antihyperglycemic, nephroprotective and antimicrobial. In the present investigation sequentially extracts of various plant parts of M. dioica, and its tissue cultures were analyzed for their antioxidant potential. Standard protocols of scavenging effect on the DPPH free radical, Haemoglobin glycosylation Assay, Ferric Reducing Antioxidant Power, Nitric oxide and Lipid Peroxidation Assay were performed with some modifications. The fractionated extractives using different solvents analyzed for their antioxidant potential, the DCF of fruits with DPPH, MF of callus with HG and MF of stem with FRAP assays showed the highest AE. Elicitation increased the AE from 85 to173% in various treated samples. Effect of salt and Vitamin C gave significant blockage that increased with time treatment. Effect of various elicitors (sodium nitroprusside and salicylic acid), ascorbic acid and sodium chloride in different concentrations and time period on AE (antiradical efficiency) was also investigated. Results were expressed as AE, which is 1000/ IC50 in case of DPPH while in FRAP it was expressed as μM/l/g. M. dioica showed that this plant is a potent antioxidant agent. It is able to increase cellular survival by reducing intracellular lipid and protein oxidation levels and ROS scavengers.