Zeitschrift für Fischerei und Aquakultur
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Microsatellite Techniques in Guam’s Specific-Pathogen-Free Penaeus vannamei Stock: Genetic Variance and Parentage Identification

Hui Gong Jiang*, Jarupan Channarong, Lertluk Ngernsiri, Akarapong Swatdipong

A microsatellite DNA marker technique was used to monitor specific-pathogen-free Penaeus vannamei (white shrimp) stock in the hatchery of the University of Guam, mainly for assessment of genetic diversity and identification of the parentage for two consecutive generations. A panel of 16 loci was selected to analyze 36 families of P. vannamei, comprising of a total of 1,152 individual shrimp samples. The families showed high genetic variation. The average number of alleles per locus was 10.625 for the parents and 10.052 for their offspring. The average observed heterozygosity declined slightly from 0.891 in the parents to 0.813 in the offspring. Similarly the expected heterozygosity was 0.804 among the parents and 0.792 for their progeny. The inbreeding coefficient was -0.107 for the parents but -0.026 for the offspring. These two generations did not differ significantly in any foregoing measures (p>0.05). CERVUS and COLONY confirmed that any 12 loci from the panel could deliver 100% correct parentage identification when the genotyping error rate was set as 0.01.