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Nuclear Magnetic Coupled Fast Radio Burst Induced Upregulation of Hsp70 in Regeneration of Human Chondrocytes - A Sham Control Study

Pillai VK, Shingati Muhammed Hashim, Manasa Nune, Gopal Pande

Manipulating the cell’s inherent vulnerabilities to induce intrinsic changes and re-engineer diseased tissues to
drive regeneration is a domain creating significant therapeutic impact through translational medicine. Articular
cartilage injuries are recurrently experienced due to trauma, mechanical stress and age-related deterioration.
Prevailing mend methods have failed to yield reliable or lasting results. Lack of suitable models that mimic the
cellular and extra cellular matrix properties of hyaline cartilage has been one of the reasons for this deficiency.
We investigated and observed, joint tissue obtained during total knee replacement surgeries from 7 discrete
patients presenting with osteoarthritis and the commonly associated symptoms of inflammation and pain. We used
modulated “Nuclear Magnetic Coupled Fast Radio Burst or simply Fast Radio Bursts”, a new modality that seems
to trigger the cellular signaling required by the articular cartilage tissue, to mend and regenerate by upregulation
of Heat Shock Protein 70. Fast Radio Bursts are high energy, short electromagnetic bursts, in which both electric
and magnetic components of the electromagnetic signals are “circularly” polarized. Fast Radio Bursts are
produced when a radio signal is traveling through a powerful instantaneous magnetic field on its path to the target.
In this Sham controlled study, up-regulation of Hsp 70 protein, to establish its role in an in vitro model was
designed to expose 2-Dimensional and 3-Dimensional cultures to Fast Radio Bursts, and compared to a Sham
Control, under identical conditions but without exposing Sham Control culture to fast radio bursts. The 2D and
3D reconstructed cartilage tissues were then assessed in both groups. Experiments were conducted to characterize
the 2D and 3D cultures to confirm total collagen, fibrillar collagen and proteoglycans, additionally;
immunofluorescence and cell viability assay was performed to identify specific bio markers like Collagen 1,
Collagen II, Aggrecan, Cell surface Adhesion factor, Hsp70 and cell viability. It could be established in this study
that 2D cultures grown in newly defined media and exposed to Fast Radio Burst signals, when compared to 2D
cultures grown as Sham culture showed more chondrocyte specific markers and viable matrix properties. In 3D
cultures grown similarly also showed better deep layer properties compared to the 3D cultures grown in sham
culture. Thus modulated Fast Radio Bursts exposure could play a significant role in specific protein up/down
regulation in tissue regeneration.