Zeitschrift für klinische und zelluläre Immunologie
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Phototoxic Assesment of Benzanthrone and Anthracene by Using NIH-3t3 and L-929 Cell Lines

Varun Tobit, O. P. Verma, P. W. Ramteke and R. S. Ray

The Polycyclic Aromatic Hydrocarbons (PAHs) are the most widespread organic environmental pollutants. PAHs are found in motor vehicle exhaust. Some PAHs are found in medicines, dyes, plastics, pesticide, naphthalene balls and wood preservatives. The compounds under the presences of Sun light generally activated and inhibited the cell growth. Hence the present investigation was focused on the maintenances and culturing of NIH-3T3 and L-929 cell lines derived from mouse fibroblast as well as the phototoxicity assessment of PAHs compounds (Anthracene and Benzanthrone) in these cell lines. Monolayer culture of cell lines was grown in DMEM culture medium. Live cells were attached in cryovial check surface and dead cell was mixed in the medium and rise up on the upper layer on the medium. The result showed that the singlet oxygen (¹O₂) generation by benzanthrone (5-50 ppm) and Anthracene (20-50 ppm) showed the phototoxicity under the exposure of UV-A (5.76 J/cm²), UV-B (2.16 J/cm²) and sunlight 60 min. Rose Bengal (50 ppm) was used as the positive control. Benzanthrone generated the more amount of singlet oxygen as compared to Anthracene while Benzanthrone generated more superoxide as compared to Anthracene.