Zeitschrift für Nanomedizin und biotherapeutische Entdeckung
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Phyto-Synthesized Silver Nanoparticles: A Potent Mosquito Biolarvicidal Agent

Hemant P Borase, Chandrashekhar D Patil, Rahul B Salunkhe, Chandrakant P. Narkhede, Bipinchandra K Salunke and Satish V Patil

Mosquito transmit diseases like malaria, dengue accounted for global mortality and morbidity with increased resistance to common insecticides. In the present study silver nanoparticles (AgNPs) were synthesized from aqueous leaves extracts of four plant species (Jatropha gossypifolia, Euphorbia tirucalli, Pedilanthus tithymaloides and Alstonia macrophylla) and there effects on IInd and IVth instars larvae of Aedes aegypti and Anopheles stephensi were evaluated. Synthesized AgNPs were characterized by UV-Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), particle size distribution and zeta potential analysis. IInd and IVth instars larvae of A. aegypti and A. stephensi were exposed to varying concentrations of AgNPs synthesized from plants under investigation (0.625 to 20 ppm) for 24 hours, which revealed larvicidal activity of AgNPs. With LC50 values of 3.50 to 7.01 ppm against IInd instar and 4.44 to 8.74 ppm against IVth instar larvae of A. aegypti and 5.90 to8.04 ppm for IInd instar, 4.90 to 9.55 ppm against IVth instar of A.  stephensi. Results obtained from this study presentbiosynthesized silver nanoparticles as novel biolarvicidal agent and can be used along with traditional insecticides as approach of Integrated Pest Management (IPM).