Zeitschrift für Chromatographie und Trenntechniken
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Quantification of Allethrin using HPTLC from Annona Squamosa (Custard Apple)

Shardul Kulkarni, Prafulla Chandra Tekale, Ratnakar Lanjewar, Swati Joshi, Kailas Chitalkar, Santosh Pathare and Rajale SN

Annona squamosa (Custard Apple) is delicious fruit and it is traditionally used for curing a variety of ailments such as Ulser, dysentery, Antiovulatory, hair problems, burning sensation, hysteria (fearful state of mind) and fainting spells. There are no reports of quantification of Allthrine from this plant. Hence a HPTLC densitometric method has been developed and validated for quantification of this marker compound. Allethrine was quantified from methanolic extract using the solvent system of Petroleum ether: Diethyl ether (7: 3, v/v). The method was validated using ICH guidelines in terms of precision, repeatability and accuracy. Linearity range for Allethrine was 40–320 ng/spot and its content was 0.01046% w/w in peel and 0.01042% w/w in seed. This simple, precise and accurate method gave good resolution from other constituents of extract. It can be adopted for routine quality control of herbal material and formulations containing Annona squamosa (Custard Apple).