Klinische und experimentelle Kardiologie
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Abstrakt

Relationship of Tumor Necrosis Factor-α 308 (G/A) Gene Polymorphism with Risk of Coronary Artery Disease in Hemodialysis Patients

Navid Kalani, Zeinab Fattahjahromi, Aghdas Shadmehr

Background: Prevalence and incidence of chronic renal failure is increasing and it has been considered as a threading factor in the worldwide. Cardiovascular diseases has been considered as the cause of more than half of death in hemodialysis patient. It has been shown that systemic inflammation has a key role in this issue. TNFα is a mediator cytokine of inflammation response which has a key role in pathophysiology of vascular disease caused by metabolism of lipids and obesity and insulin resistance. The aim of this study was to assess the association of polymorphism -308G/A gene TNF alpha and severity and vulnerability of cardiovascular disease in hemodialysis patient.

Materials and methods: In this case control study, we had three groups of participants; 41 hemodialysis patient with cardiovascular risk factors and 41 hemodialysis patient without cardiovascular risk factors and also 41 healthy participants without cardiovascular risk factors as control group. DNA extraction was done with the specific Kit for the extraction of DNA. PCR was used for detection of polymorphism -308G/A and then Nocl enzyme was added to each polymorphism for slicing and then electrophoresis was used for confirmation of enzyme slicing. Date were gathered and analyzed with SPSS v 15.

Results: There is no significant difference between the 3 group regarding genotype AA distribution and frequency of Allel A (p value>0.05).

Conclusion: The results of this study demonstrate that there is not any association between this polymorphism and cardiovascular risk in Iranian population. Other studies with larger samples are beneficent in order to detect the role of this polymorphism.