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A Novel Limosilactobacillus reuteri Strain Byun-re-01 Exhibits Promising Probiotic Properties for Further Use as Preservatives: An In Vivo and In Silico Study

Sukchan Lee

Antibiotics had been a pivotal substance to be supplemented in the food for poultry flock with a main purpose to improve their yields in order to gain profits. However, its long-term use costs some adverse effects such as resistance and tolerance. Hence, a new platform is of urgency to figure out. We discovered that our probiotic bacteria, which through the whole-genome sequencing was identified as Limosilactobacillus reuteri strain Byun-re-01 (L. reuteri Byun- re-01), exhibited promising biological properties to substitute the existing use of antibiotics. Oral administration of L. reuteri Byun-re-01 was shown to provide local protection by producing anti-inflammatory cytokines mainly IFN-β and IFN-γ in mRNA levels. In addition, they suppressed the expression of viral capsid mRNA of MNV indicating their antiviral activity. They were not detected to translocate to distant organs but to reside and colonize only within the gut. The ALT/AST levels in the serums of mice receiving L. reuteri Byun-re-01-treatment were observed to be at normal range suggesting that no functional damages of the mice’s livers were detected. Overall, our results showed favorable profiles of antiviral-carrying probiotics in L. reuteri Byun-re-01 that render it a promising candidate of non- antibiotic preservatives.