Fortschritte in der Milchforschung
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Abstrakt

Identification of Bioactive Peptides in Goat Milk and Their Health Application

Garima Sharma*, Pramod Kumar Rout, Rakesh Kaushik and Gajendra Singh

We have identified the bioactive peptides in goat milk using two dimensional gel electrophoresis and mass spectrometry. The milk samples were collected from three healthy breeds of goats at CIRG farms. The milk proteins were isolated using simple centrifugation and were evaluated on the basis of presence of other proteins variants using SDS PAGE and two dimensional gel electrophoresis. The protein profiles of all the milk proteins were same showing the presence of all the major caseins proteins and whey proteins whereas the two dimensional gel electrophoresis resulted gels has shown the presence of various protein spots. These protein spots were analysed under mass spectrometry and found biologically active peptides dipeptidyl peptidase, transcription factor A, Interleukin 12 subunit, oligodendrocyte transcription factor 2. In our study we have reported the biological significance of these peptides identified and their role in human health such as mitochondrial disease, brain malignancies, signaling processes of cytokines and help in generating cytotoxic lymphocyte helps enhance immunity.