Zeitschrift für Krebswissenschaft und -forschung
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Curcuminoids Suppress the Number of Transformed-Hepatocytes and Ki67 Expression in Mice Liver Carcinogenesis Induced by Diethylnitrosamine

Atmodjo WL, Larasati YO, Isbandiati D and Mathew G

Background: Curcuminoids has been reported to have strong anti-oxidant and anti-inflammatory effects, however the effect of curcuminoids to hepatocytes and their proliferation in DEN (Diethylnitrosamine) induced mice liver carcinoma model is still not yet been studied in detail.
Objection: To evaluate the effects of curcuminoids-containing diets in liver carcinoma mice induce with Diethylnitrosamine
Methods: Forty of 5 weeks old, male Balb/C mice were administered DEN as an injection and fed with basal diet; and another group injected with DEN and fed with 0.2% curcuminoids-containing diet. Dose of injection DEN is 75 mg/kg BW/week for 3 weeks, continued with 100 mg/kg BW/week for 3 continuous weeks. Five animals in each group were sacrificed at 20 and 32 weeks after the first injection of DEN. The liver sections were stained with HE (Hematoxylin Eosin) and antibody anti Ki67. The number of transformed-hepatocytes and Ki67-positive cells were counted randomly in high-powered fields.
Results: The liver of group 3 showed disruption of hepatic architecture with dilated sinusoids, lymphocytes infiltration, hydrophic and fatty degeneration towards necrotic hepatocytes. Hepatocytes were found pleomorphic and large in size with hyperchromatic nuclei and prominent nucleoli.
Conclusion: Our study has shown that curcuminoids diet suppressed increasing number of transformed- hepatocytes and Ki67-positive cells significantly (p<0.05).