Enzymtechnik
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Abstrakt

Dynamics of Mitochondrial Nucleoid Proteins Involved in Development of Cotyledon Mitochondria during Mung Bean Seed Germination

Ning Cheng, Yih-Shan Lo, Na-Sheng Lin*, Hwa Dai*

Mitochondrial DNA (mtDNA) protein complexes, called mitochondrial-nucleoids (mt-nucleoids), are dynamic and redistribute during mitochondria propagation and gene expression. These proteins involve in mtDNA replication, transcription, maintenance and repair. In this study, we analyzed mt-nucleoid proteins in isolated mt-nucleoids from cotyledons at different developmental ages. We aimed to understand the changes in mt-nucleoids associated protein during cotyledon development. We found that some transcription/translation factors pre-exist in nucleoids of dormant seed mitochondria. High numbers of metabolic proteins co-purified with mtDNA appeared in dormant seeds and developmental cotyledon mitochondria. This report provides some idea about the dynamic of mt-nucleoid proteins along with cotyledon mitochondrial development during seed germination.