Pharmazeutische Analytische Chemie: Offener Zugang
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Abstrakt

Gleichzeitige Bestimmung von vier polaren Indexbestandteilen durch HILIC-ESIMS/ MS zur Qualitätskontrolle von Guizhi Fuling Wan

Zhiran Zhang, Jin Wang, Wen Yao, Jingju Men, Xiaobo Wang und Longshan Zhao

A rapid and accurate method was developed and validated for the analysis of four index polar constitutes in Chinese medicinal preparation Gui-zhi-fu-ling Wan by microwave-assisted extraction coupled with hydrophilic interaction liquid chromatography-tandem mass spectrometry (MAE-HILIC -MS/MS). The chromatographic separation was performed on a Waters HILIC column (100 mm × 1.7 mm, 2.1 µm) with gradient elution using 0.1% formic acid aqueous solution (A) and acetonitrile (B) at a flow rate of 0.2 mL/min. All the compounds showed good linearity. The method provided good accuracy with perfect recoveries and precision, which has been successfully applied to the simultaneous identification and determination of 4 index compounds in a single run for the quality control of Gui-zhi-fu-ling Wan.